| Date |
Topic |
Weinberg |
Articles |
Presenter |
Assignment |
| Sept 3 |
Introduction to course |
— |
— |
Profs. Seligman and Hamlett |
— |
| |
| Sept 8 |
Introduction to cancer |
2.1
2.4–2.11
11.1–11.5 |
— |
Prof. Seligman
PowerPoint |
— |
| Sept 10 |
Viruses & cancer — an introduction |
3.1–3.3
3.5–3.12 |
Rous, P. 1911. Transmission of a malignant new growth by means of a cell-free filtrate. JAMA 56: 471. [pdf]
Martin, G. S. 1970. Rous sarcoma virus: A function required for the maintenance of the transformed state. Nature 227: 1021-1023. [pdf]
Stehelin, D., H.E. Varmus, J.M. Bishop, and P.K. Vogt. 1976. DNA related to the transforming gene(s) of avian sarcoma viruses is present in normal avian DNA. Nature 260: 170-173. [pdf]
Martin, G.S. 2001. The hunting of the Src. Nat Rev Mol Cell Biol 2: 467-475 [html | pdf] |
Prof. Hamlett
PowerPoint |
3 questions for Weinberg
No assignment for articles |
| |
| Sept 15 |
Oncogenes |
4.2–4.3 |
Shih, C., and R.A. Weinberg. 1982. Isolation of a transforming sequence from a human bladder carcinoma cell line. Cell 29: 161-169. [pdf] |
Sam Cook
PowerPoint
Feedback |
3 questions for Weinberg
Questions for article |
| Sept 17 |
Oncogenes (cont) |
4.4–4.6 |
Tabin, C.J., S.M. Bradley, C.I. Bargmann, R.A. Weinberg, A.G. Papageorge, E.M. Scolnick, R. Dhar, D.R. Lowy, and E.H. Chang. 1982. Mechanism of activation of a human oncogene. Nature 300: 143-149. [pdf] |
Ray Addante
PowerPoint
Feedback |
3 questions for Weinberg
Summary for article |
| |
| Sept 22 |
Oncogenic pathways |
5.1–5.5
5.9, 6.1 |
Collett, M.S. and R.L. Erikson. 1978. Protein kinase activity associated with the Avian Sarcoma Virus src gene product. PNAS 75: 2021-2024. [pdf]
Bryant, D., and J.T. Parsons. 1983. Site-directed point mutation in the src gene of Rous Sarcoma Virus result in an inactive src gene product. J. Virol. 45: 1211-1216. [pdf | Fig2 | Fig3 | Fig4]
Smith, M.R., S.J. DeGudicibus, and D.W. Stacey. 1986. Requirement of c-ras proteins during viral oncogene transformation. Nature 320: 540-543. [pdf] |
Group presentations & class discussion |
3 questions for Weinberg
Group Presentation Instructions |
| Sept 24 |
Introduction to tumor suppressors |
7.1–7.6 |
Leppert, M., M. Dobbs, P. Scambler, P. O’Connell, Y. Nakamura, D. Stauffer, S. Woodward, R. Burt, J. Hughes, E. Gardner, M. Lathrop, J. Wasmuth, J.-M. Lalouel, and R. White. 1987. The gene for familial polyposis coli maps to the long arm of chromosome 5. Science 238: 1411-1413. [html | pdf]
Fearon, E. R., K. R. Cho, J. M. Nigro, S. E. Kern, J. W. Simons, J. M. Ruppert, S. R. Hamilton, A. C. Preisinger, G. Thomas, K. W. Kinzler, B. Vogelstein. 1990. Identification of a chromosome 18q gene that is altered in colorectal cancers. Science 247: 49-56. [html | pdf] |
Prof. Seligman
PowerPoint |
3 questions for Weinberg
No assignment for articles |
| |
| Sept 29 |
Tumor suppressor genes |
7.7–7.13 |
Knudson, A. G. 1971. Mutation and cancer: A statistical study of retinoblastoma. PNAS 68: 820-823. [pdf] |
Andrea Cohen
PowerPoint
Feedback |
3 questions for Weinberg
Questions for article |
| Oct 1 |
More Tumor suppressor genes |
— |
Cavenee, W.K., T.P. Dryja, R.A. Phillips, W.F. Benedict, R.Godbout, B.L. Gallies, A.L. Murphree, L.C. Strong, and R.L. White. 1983. Expression of recessive alleles by chromosomal mechanisms in retinoblastoma. Nature 305: 779-784. [pdf | Clearer gel photos are available on Sakai] |
Kendra Henderson
PowerPoint
Feedback |
Read Cavenee et al. (1983)
Write a News Release for Lee et al. (1987) |
| Lee, W.-H., R. Bookstein, F, Hong, L.-J. Young, J.-Y. Shew, and E.Y.-H. P. Lee. 1987. Human retinoblastoma susceptibility gene: Cloning, identification, and sequence. Science 235: 1394-1399. [html | pdf] |
Allison Dubner
PowerPoint
Feedback |
| |
| Oct 6 |
Introduction to cell cycle |
8.1–8.4
8.12 |
Hartwell, L. H. 1991. Twenty-five years of cell cycle genetics. Genetics 129: 975-980. [pdf]
Hartwell, L. H., J. Culotti, J. R. Pringel, and B. J. Reid. 1974. Genetic control of the cell division cycle in yeast. Science 183: 46-51. [html | pdf]
Lee, M. G., and P. Nurse. 1987. Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature 327: 31-35. [pdf] |
Prof. Seligman
PowerPoint |
3 questions for Weinberg
No assignment for articles |
| Oct 8 |
Cell cycle |
8.6–8.8 |
Nurse, P., and P. Thuriaux. 1980. Regulatory genes controlling mitosis in the fission yeast Schizosaccharomyces pombe. Genetics 96: 627-637. [pdf] |
Doug Campbell
PowerPoint
Feedback |
3 questions for Weinberg
Summary for article |
| |
| Oct 13 |
Checkpoints |
8.9-8.13 |
Weinert, T. A., and L. H. Hartwell. 1988 The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae. Science 241: 317-322. [html | pdf] |
Doug Farquhar
PowerPoint
Feedback |
3 questions for Weinberg
Summary for article |
| Oct 15 |
MIDTERM EXAM
|
| |
| Oct 20 |
FALL BREAK - no class
|
| Oct 22 |
Checkpoints (cont) |
12.11-12.12 |
Cahill, D. P., C. Lengauer, Yu, G. J. Riggins, J. K. V. Willson, S. D. Markowitz, K. W. Kinzler, and B. Vogelstein. 1998. Mutations of mitotic checkpoint genes in human cancers. Nature 392: 300-303 [html | pdf] |
Michael Klein
PowerPoint
Feedback |
3 questions for Weinberg
Summary for article |
| |
| Oct 27 |
DNA tumor viruses & p53 |
3.4
9.1–9.4 |
Levine, A. J., C. A. Finlay, and P. W. Hinds. 2004. P53 is a tumor suppressor gene. Cell 116: S67-S69. (Commentary) [pdf]
Crawford, L. V., D. C. Pim, E. G. Gurney, P. Goodfellow, and J. Taylor-Papadimitriou. 1981. Detection of a common feature in several human tumor cell lines—A 53,000-Dalton Protein. Proc. Nat. Acad. Sci. USA 78[Part 2: Biological Sciences]: 41-45. [pdf | High-resolution figures in JSTOR: Fig1 | Fig2 | Fig3 | Fig4 | Fig5 | Fig6]
Finlay, C. A., P. W. Hinds, and A. J. Levine. 1989. The p53 proto-oncogene can act as a suppressor of transformation. Cell 57: 1083-1093. [pdf] |
Prof. Hamlett |
3 questions for Weinberg
No assignment for articles |
| Oct 29 |
p53 |
9.5–9.7 |
Baker, S. J., S. Markowitz, E. R. Fearon, J. K. V. Willson, and B. Vogelstein. 1990. Suppression of human colorectal carcinoma cell growth by wild-type p53. Science 249: 912-915. [html | pdf] |
Andrea Cohen
PowerPoint
Feedback |
3 questions for Weinberg
Write a News Release for Donehower et al. (1992) |
| Donehower, L. A., J. Harvey, B. L. Slagle, M. J. McArthur, C. A. Montgomery, Jr., J. S. Butel, and A. Bradley. 1992. Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumors. Nature 356: 215-221. [pdf | Some gels are clearer in these figures on Sakai] |
Doug Campbell
PowerPoint
Feedback |
| |
| Nov 3 |
p53 (cont) |
9.8–9.10
9.13–9.14 |
Lowe, S. W., E. M. Schmitt, S. W. Smith, B. A. Osborne, and T. Jacks. 1993. p53 is required for radiation-induced apoptosis in mouse thymocytes. Nature 362: 847-849. [pdf] |
Doug Farquhar
PowerPoint
Feedback |
3 questions for Weinberg
Questions for the two articles |
| Polyak, K., Y. Xia, J. L. Zweier, K. W. Kinzler, and B. Vogelstein. 1997. A model for p53-induced apoptosis. Nature 389: 300-305. [html | pdf | Fig1 | Fig2 | Fig3 | Fig4] |
Sam Cook
PowerPoint
Feedback |
| Nov 5 |
p53 (cont) |
9.11–9.12 |
Venkatachalam S., Y. P. Shi, S. N. Jones, H. Vogel, A. Bradley, D. Pinkel, and L. A. Donehower. 1998. Retention of wild-type p53 in tumors from p53 heterozygous mice: Reduction of p53 dosage can promote cancer formation. EMBO J. 17: 4657-4667. [html | pdf]
Lang, G., T. Iwakuma, Y. Suh, G. Liu, V. Rao, J. Parant, Y. Valentin-Vega, T. Terzian, L. Caldwell, and L. Strong. 2004. Gain of function of a p53 hot spot mutation in a mouse model of Li-Fraumeni Syndrome. Cell 119: 861-872. [html | pdf] |
Class discussion |
No questions for Weinberg
Discussion assignment |
| |
| Nov 10 |
Introduction to telomeres and telomerase |
10.1–10.2
10.4–10.6
|
Greider, C. W., and E. H. Blackburn. 1985. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43: 405-413. [html | pdf]
Yu, G., J. D. Bradley, L. D. Attardi, and E. H. Blackburn. 1990. In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs. Nature 344: 126-131. [pdf]
Shay, J. W., and W. E. Wright. 2000. TIMELINE: Hayflick, his limit, and cellular ageing. Nat. Rev. Mol. Cell Biol. 1: 72-76. [html | pdf]
Kim, N. W., M. A. Piatyszek, K. R. Prowse, C. B. Harley, M. D. West, P. L. C. Ho, G. M. Coviello, W. E. Wright, S. L. Weinrich, and J. W. Shay. Specific association of human telomerase activity with immortal cells and cancer. Science 266: 2011-2015. [JSTOR: html | pdf] |
Prof. Hamlett |
3 questions for Weinberg
No assignment for articles |
| Nov 12 |
Immortality |
10.7-10.11 |
Blasco, M.A., H-W. Lee, M.P. Hande, E. Samper, P.M. Lansdorp, R.A. DePinho, and C.W. Greider. 1997. Telomere shortening and tumor formation by mouse cells lacking telomerase RNA. Cell 91: 25-34. [html | pdf] |
Michael Klein
PowerPoint
Feedback |
3 questions for Weinberg
Summaries for each of the two articles |
| Artandi, S. E., S. Chang, S.L. Lee, S. Alson, G. J. Gottlieb, L. Chin, and R. A. DePinho. 2000. Telomere dysfunction promotes non-reciprocal translocations and epithelial cancers in mice. Nature 406: 641-645. [html | pdf | Fig1 | Fig2 | Fig3] |
Raymond Addante
PowerPoint
Feedback |
| |
| Nov 17 |
Multi-step carcinogenesis |
11.9-11.11 |
Hahn, W. C., C. M. Counter, A. S. Lundberg, R. L. Beijersbergen, M. W. Brook, and R. A. Weinberg. 1999. Creation of human tumour cells with defined genetic elements. Nature 400: 464-468. [html | pdf | Fig1 | Fig2 | Fig3 | Fig4] |
Jordan Eboreime
PowerPoint
Feedback |
3 questions for Weinberg
Summary for article
|
| Introduction to mutators |
12.3-12.8 |
Hoess, R. H., and R. K.,Herman. 1975. Isolation and characterization of mutator strains of Escherichia coli K-12.J. Bacteriol. 122(2): 474-484. [pdf]
Nghiem, Y., M. Cabrera, C. G. Cupples, and J. H. Miller. 1988. The mutY gene: A mutator locus in Escherichia coli that generates GC  TA transversions. PNAS 85: 2709-2713. [pdf]
Michaels, M.L., C. Cruz, A. P. Grollman, and J. H. Miller. 1992. Evidence that MutY and MutM combine to prevent mutations by an oxidatively damaged form of guanine in DNA. PNAS 89: 7022-7025. [pdf]
Reenan, R. A., and R. D. Kolodner. 1992. Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins. Genetics 132: 963-973. [pdf]
Strand, M., T. A. Prolla, R. M. Liskay, and T. D. Petes. 1993. Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair. Nature 365: 274-276. [pdf] |
Prof. Seligman
PowerPoint |
3 questions for Weinberg
No assignment for articles |
| Nov 19 |
Genomic integrity |
12.9-12.10 |
Fishel, R., M. K. Lescoe, M. R. S. Rao, N. G. Copeland, N. A. Jenkins, J. Garber, M. Kane, and R. Kolodner. 1993. The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer. Cell 75: 1027-1038. [pdf] |
Allison Dubner
PowerPoint
Feedback |
3 questions for Weinberg
Summary for article |
| |
| Nov 24 |
Mutators and cancer |
— |
Nicolaides, N. C., S. J. Littman, P. Modrich, K. W. Kinzler, and B. Vogelstein. 1998. A naturally occurring hPMS2 mutation can confer a dominant negative mutator phenotype. Mol. Cell. Biol. 18: 1635-1641. [html | pdf | Fig1 | Fig2 | Fig3 | Fig4 | Fig5 | Fig6] |
Kendra Henderson
PowerPoint
Feedback |
Summary for article |
| Nov 26 |
Pre-Thanksgiving Break - no class
|
| |
| Dec 1 |
Stem cells and cancer |
11.6., 12.2 |
Al-Hajj, M. S. Wicha, A. Benito-Hernandez, S. J. Morrison, and M. F. Clarke. 2003. Prospective identification of tumorigenic breast cancer cells. PNAS 100(7): 3983-3988. [html | pdf] |
Jordan Eboreime
PowerPoint
Feedback |
3 questions for Weinberg
Summary for article |
| Dec 3 |
— |
— |
Optional consultations on Final Projects |
|
— |
| |
| Dec 8 |
— |
— |
Final project presentations |
Raymond Addante
Andrea Cohen
Doug Farquhar
|
— |
| Dec 10 |
— |
— |
Final project presentations |
Michael Klein
Doug Campbell
Jordan Eboreime |
— |
Dec 16
2 pm |
— |
— |
Final project presentations |
Allison Dubner
Kendra Henderson
Sam Coook
|
— |
